Human Cytomegalovirus miR-US5-2 Downregulation of GAB1 Regulates Cellular Proliferation and UL138 Expression through Modulation of Epidermal Growth Factor Receptor Signaling Pathways
Regulation of the epidermal growth factor (EGF) receptor (EGFR) signaling is essential for the replication of human cytomegalovirus (HCMV) and the latency and reactivation in CD34 + hematopoietic progenitor cells. HCMV microRNAs (miRNAs) provide a means to modulate the signal is activated by EGF through targeting EGFR signaling pathway components.
Here, we show that miR-US5-2 HCMV immediate critical downregulates EGFR GAB1 adapter proteins that mediate sustained activation and signals through phosphatidylinositol 3-kinase (PI3K) and MEK / extracellular signal-regulated kinase (ERK) pathway and cell proliferation in response to EGF. UL138 HCMV expression is regulated by the transcription factor early growth response gene 1 (EGR1) downstream of EGFR-induced MEK / ERK signaling. We show that by targeting GAB1 and smoothes the MEK / ERK signaling, mir-US5-2 indirectly regulating the expression EGR1 and UL138, which implicates critical miRNA regulation latency.IMPORTANCE HCMV Human cytomegalovirus (HCMV) causes significant illness in immunocompromised individuals, including transplant patients.
HCMV establishes latency in the hematopoietic stem cells in the bone marrow. The mechanisms that regulate the latency and reactivation of viral replication is complex and not fully understood. HCMV-encoded miRNAs are small regulatory RNA that reduces expression of the protein. In this study, we found that HCMV miRNA miR-US5-2 targeting the epidermal growth factor receptor (EGFR) protein GAB1 adapter that directly affect downstream cellular signaling pathways activated by EGF.
As a result, mir-US5-2 block EGF-mediated proliferation of human fibroblasts. early growth response gene 1 (EGR1) is a transcription factor activated by EGFR signaling that regulates the expression of HCMV UL138. We show that miR-UL138 US5-2 regulates expression via downregulation GAB1-mediated signaling pathways that lead to the expression of EGR1. These data demonstrate that miR-US5-2, through downregulation of GAB1, can play an important role during the reactivation from latency by reducing the proliferation and expression of UL138.
Human Cytomegalovirus miR-US5-2 Downregulation of GAB1 Regulates Cellular Proliferation and UL138 Expression through Modulation of Epidermal Growth Factor Receptor Signaling Pathways
early diagnosis and successful treatment of cytomegalovirus peritonitis in children with primary nephrotic syndrome: a case series and review of the literature
Cytomegalovirus (CMV) is a major pathogen in immunocompromised population and CMV infections in immunocompromised patients cause of morbidity and mortality are quite large. Common clinical manifestations of CMV infection is pneumonia, hepatitis, colitis and so on, while CMV without intestinal perforation peritonitis rarely occurs. Reviewing the literature, CMV peritonitis in patients with nephrotic syndrome (NS) has not been reported. Only four cases of peritonitis CMV without bowel perforation reported in adults with other illnesses.
Two cases were diagnosed by reverse-transcription polymerase chain reaction (RT-PCR) of ascites while two other cases with histopathological examination of peritoneal tissue. We report four cases of primary nephrotic syndrome complicated with CMV peritonitis. Four cases were all diagnosed by RT-PCR from ascites (659-455000 copies / mL).
Description: A sandwich quantitative ELISA assay kit for detection of Human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) in samples from tissue homogenates or other biological fluids.
Human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) in samples from tissue homogenates or other biological fluids.
Human Aminoadipate Semialdehyde Phosphopantetheinyl Transferase (AASDHPPT) ELISA Kit
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, IHC, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.ELISA:1/40000
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200
Description: A polyclonal antibody against AASDHPPT. Recognizes AASDHPPT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200
Description: The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia.
We mainly discuss the diagnosis and treatment of CMV without intestinal perforation peritonitis.Human cytomegalovirus (HCMV) is a double-stranded DNA virus widely infected humans. Circular RNAs (circRNAs) is a non-coding RNA with most functions remain unknown, and the effects of HCMV infection in the host circRNA productive transcription remains unclear. In this study, we profiled 283 hosts a significant circRNAs amended by the productive infection of HCMV in human embryonic lung fibroblast (helf) by RNA deep sequencing and bioinformatics analysis. Among other things, circSP100, circMAP3K1, circPLEKHM1, and circTRIO validated for transcription and their sequence.
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