Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection

Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection

Objective: To investigate and compare the clinical features of cytomegalovirus (CMV) retinitis after solid organ transplant (SOT) and hematopoietic stem cell transplantation (HSCT) and to define poor prognostic factors.


Methods: Patients consult ophthalmology department for CMV viremia after transplantation between March 2008 and February 2018 and were followed for more than 6 months were analyzed. medical records regarding demographics, serology, and characteristics of the comparison between the SOT and HSCT. Factors associated with poor visual outcome was determined by logistic regression.


Results: CMV retinitis developed in 11.3% of patients with CMV viremia after transplantation. In the group SOT (25 points / 18 patients) and the HSCT group (33 eyes / 21 patients), CMV retinitis occurred in 5.8 months and 3.7 months post-transplantation, respectively. Mortality was significantly higher in the HSCT group (52.4% vs 5.6%, P <0.001). During an average of 11.7 months of follow-up, visual acuity tends to be exacerbated (P = 0.087) despite antiviral treatment, which is especially important in SOT group (P = 0.028). Six eyes (10.3%) due to retinal detachment underwent vitrectomy, which most (5 eyes) should be in the SOT. Multivariate logistic regression analysis showed that the concurrent presence of CMV disease (OR = 14.11, P = 0.009) and foveal involvement (OR = 114.85, P = 0.001) is a poor prognostic factor.


Conclusion: Clinical manifestations of CMV retinitis differ between groups HSCT and SOT. CMV disease simultaneously and foveal involvement is associated with poor visual outcome after transplantation CMV retinitis.


Human cytomegalovirus (HCMV) is a leading infectious agent causing birth defects. HCMV infection risk to the fetus in pregnant women who receive immunosuppressive agents is unknown. We describe two cases of pregnant women with pre-conception evidence of protective immunity HCMV receiving azathioprine for ulcerative colitis or systemic lupus erythematosus.

Both women reactivate HCMV infections and transmitted to the fetus. One newborn showed unilateral hearing deficit and brain disorders while others are asymptomatic. The mother of a newborn baby has symptoms of low levels of CD4 + T cells and HCMV-specific total blood. Women who receive immunosuppressive agents decent information about the risk of congenital HCMV infection and should be monitored for HCMV infection during pregnancy. their infants should be screened for congenital HCMV infection.

 Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection
Human Cytomegalovirus Influences Host circRNA Transcriptions during Productive Infection

The frequency of congenital cytomegalovirus infection in newborn babies in the State of Sao Paulo, 2010-2018

Human cytomegalovirus (HCMV) infection remains a neglected public health problem. The purpose of this study was to evaluate the frequency of congenital HCMV infection in newborns up to 1 month in Sao Paulo State, from 2010 to 2018.

Molecular characterization of HCMV-positive samples were also made. 275 urine samples from prospective patients congenital HCMV infection were tested by real-time Polymerase Chain Reaction (qPCR). HCMV-positive samples amplified by conventional PCR targeting UL89 gene, sorted and look for mutations.

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AADACL4 3'UTR GFP Stable Cell Line

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Steady-Luc Firefly HTS Assay Kit (10x100 ml)

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AADACL4 Antibody

1-CSB-PA782584
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33R-2935 100 ug
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Arylacetamide Deacetylase-Like 4 (AADACL4) Antibody

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CD43-Luc (GFP) Lentivirus 

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CD43-Luc (Neo) Lentivirus 

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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (Puro) Lentivirus 

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Description: Pre-madelentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD43-Luc (RFP) Lentivirus 

LVP1003-R 1x107 IFU/ml x 200ul
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (Bsd) Lentivirus 

LVP1007-B 1x107 IFU/ml x 200ul
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Blasticidin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (GFP) Lentivirus 

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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (Neo) Lentivirus 

LVP1007-N 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the Neomycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (Puro) Lentivirus 

LVP1007-P 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-madelentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the puromycin selection marker under RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

CD45-Luc (RFP) Lentivirus 

LVP1007-R 1x107 IFU/ml x 200ul
EUR 552
Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the RFP selection marker under the constitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

A total of 32 (11.6%) HCMV-positive cases detected (mean Ct 30.59); the mean and median age of 10.3 and 6 days old, respectively. Children aged between 0-3 weeks HCMV has a higher detection rate (84.4%; 27/32). UL89 gene successfully sorted into two samples, both classified as human betaherpesvirus 5. Not Specified associated resistance mutations were identified. A routine screening in newborns coupled with the genetic characterization of viral genes important key to lowering the sequel associated with congenital HCMV infection.

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