Background: Human cytomegalovirus (HCMV) causes no symptoms, but also cause congenital infection when HCMV-infected women during pregnancy, and life-threatening disease in immunocompromised patients. To better understand the mechanism of neutralization activity against HCMV, HCMV antibody titers association NT judged by antibody titers against each glycoprotein complex (gc) of HCMV.
Methods: Sera were collected from 78 healthy adult volunteers were used. Merlin HCMV HCMV clinical isolates strain and 1612 strain used in the NT test with plaque reduction test, in which both the MRC-5 fibroblasts cells and RPE-1 epithelial cells are used. Complex glycoprotein gB, gH / gL complex (gH / gL / GO and gH / gL / UL128-131A [PC]) and GM / GN selected as the target glycoprotein. 293FT cells expressed with gB, GM / gn, gH / gL / GO, or PC, is prepared and used for the measurement of antibody titers against each gc in an indirect immunofluorescence assay (IIFA). The correlation between titer IIFA for every gc and HCMV-NT titers evaluated.
Results: No significant correlation between specific titer IIFA gB and HCMV-NT titers in the epithelial cells or between GM / GN specific complex titer and titer IIFA HCMV-NT. On the other hand, there is a positive correlation statistically significant between the titer IIFA for complex gH / gL and the titer of HCMV-NT.
Conclusion: The data show that the complex of gH / gL may be a prime target for inducing activity against HCMV NT.
Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes
Based Necroptosis CRISPR screen Neuropilin-1 knockout revealed as the host factors important for the early stages of murine cytomegalovirus infection
The herpes virus is a human pathogen everywhere which causes various health complications. Currently, there is an incomplete understanding of the cellular factors that contribute to the herpes virus infection. Here, we report the antivirus necroptosis based genetic screen to identify factors of new host cells is required for β-herpes virus infection of murine cytomegalovirus (MCMV).
screen-based genome-wide CRISPR we utilized the capacity of the mutant herpes virus that triggers cell death necroptotic antivirus virus early gene expression. Vascular endothelial growth factor (VEGF) and semaphorin receptor binding Neuropilin-1 (Nrp-1) has emerged as an important determinant of MCMV infection. We found that the removal of damage early Nrp-1 virus gene expression and reduce the rate of infection in endothelial cells, fibroblasts and macrophages. Furthermore, preincubation with soluble Nrp virus-1 infection by reducing dramatically inhibit virus attachment. Thus, Nrp-1 is a major determinant of the initial phase of MCMV infection.
Description: MM-102 is an antagonist of MLL1 with IC50 value of 2.4nM [1].Mixed lineage leukemia 1 (MLL1) is a histone H3 lysine 4 (H3K4) methyltransferase.
Description: MM-102 is an antagonist of MLL1 with IC50 value of 2.4nM [1].Mixed lineage leukemia 1 (MLL1) is a histone H3 lysine 4 (H3K4) methyltransferase.
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180/Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y323
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IHC-P, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of T180
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat. This p38 antibody is for WB, IF, IHC-P, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human p38 around the non-phosphorylation site of Y182
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: A polyclonal antibody for detection of p38 from Human, Mouse, Rat, Chicken. This p38 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human p38
Description: p38 is a protein encoded by the MAPK14 gene which is approximately 41,2 kDa. p38 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling, the IL-2 pathway, toll-like receptor signalling pathways, the VEGF signalling pathway and 4-1BB pathway. This protein falls under the MAP kinase family. It acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. p38 is expressed in the brain, heart, placenta, pancreas and skeletal muscle. Mutations in the MAPK14 gene may result in patellar tendinitis and lumbosacral lipoma. STJ94878 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of p38 protein.
Cytomegalovirus (CMV) is a ubiquitous virus that infects people around the world. CMV is known to trigger thrombocytopenia, but the relationship is probably underdiagnosed since CMV infection in healthy adults usually either asymptomatic or cause mild symptoms. A systematic literature review conducted and produced 23 publications that reported in 25 patients. All hematology centers in Israel were searched for adult immunocompetent patients with CMV-related thrombocytopenia, and five new cases were identified. The average age of the combined 30 patients was 33 years (range 18-80), 73% were male, 77% presented with CMV-related symptoms, 48% had an enlarged spleen, 95% had atypical lymphocytes in the peripheral blood and 68% have increased transaminase levels.
The response rate for the first-line regimen containing steroids only 31%, while 11 patients were treated with anti-CMV agents have a response rate of 82%. In addition, four patients received receptor agonist thrombopoietin (TPO-RA) are three (75%) responded. Taken together, a typical feature of cases with thrombocytopenia must guard against CMV infection as the source.
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