Background: Human cytomegalovirus (HCMV) causes no symptoms, but also cause congenital infection when HCMV-infected women during pregnancy, and life-threatening disease in immunocompromised patients. To better understand the mechanism of neutralization activity against HCMV, HCMV antibody titers association NT judged by antibody titers against each glycoprotein complex (gc) of HCMV.
Methods: Sera were collected from 78 healthy adult volunteers were used. Merlin HCMV HCMV clinical isolates strain and 1612 strain used in the NT test with plaque reduction test, in which both the MRC-5 fibroblasts cells and RPE-1 epithelial cells are used. Complex glycoprotein gB, gH / gL complex (gH / gL / GO and gH / gL / UL128-131A [PC]) and GM / GN selected as the target glycoprotein. 293FT cells expressed with gB, GM / gn, gH / gL / GO, or PC, is prepared and used for the measurement of antibody titers against each gc in an indirect immunofluorescence assay (IIFA). The correlation between titer IIFA for every gc and HCMV-NT titers evaluated.
Results: No significant correlation between specific titer IIFA gB and HCMV-NT titers in the epithelial cells or between GM / GN specific complex titer and titer IIFA HCMV-NT. On the other hand, there is a positive correlation statistically significant between the titer IIFA for complex gH / gL and the titer of HCMV-NT.
Conclusion: The data show that the complex of gH / gL may be a prime target for inducing activity against HCMV NT.
Association of human cytomegalovirus (HCMV) neutralizing antibodies with antibodies to the HCMV glycoprotein complexes
Based Necroptosis CRISPR screen Neuropilin-1 knockout revealed as the host factors important for the early stages of murine cytomegalovirus infection
The herpes virus is a human pathogen everywhere which causes various health complications. Currently, there is an incomplete understanding of the cellular factors that contribute to the herpes virus infection. Here, we report the antivirus necroptosis based genetic screen to identify factors of new host cells is required for β-herpes virus infection of murine cytomegalovirus (MCMV).
screen-based genome-wide CRISPR we utilized the capacity of the mutant herpes virus that triggers cell death necroptotic antivirus virus early gene expression. Vascular endothelial growth factor (VEGF) and semaphorin receptor binding Neuropilin-1 (Nrp-1) has emerged as an important determinant of MCMV infection. We found that the removal of damage early Nrp-1 virus gene expression and reduce the rate of infection in endothelial cells, fibroblasts and macrophages. Furthermore, preincubation with soluble Nrp virus-1 infection by reducing dramatically inhibit virus attachment. Thus, Nrp-1 is a major determinant of the initial phase of MCMV infection.
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Cytomegalovirus (CMV) is a ubiquitous virus that infects people around the world. CMV is known to trigger thrombocytopenia, but the relationship is probably underdiagnosed since CMV infection in healthy adults usually either asymptomatic or cause mild symptoms. A systematic literature review conducted and produced 23 publications that reported in 25 patients. All hematology centers in Israel were searched for adult immunocompetent patients with CMV-related thrombocytopenia, and five new cases were identified. The average age of the combined 30 patients was 33 years (range 18-80), 73% were male, 77% presented with CMV-related symptoms, 48% had an enlarged spleen, 95% had atypical lymphocytes in the peripheral blood and 68% have increased transaminase levels.
The response rate for the first-line regimen containing steroids only 31%, while 11 patients were treated with anti-CMV agents have a response rate of 82%. In addition, four patients received receptor agonist thrombopoietin (TPO-RA) are three (75%) responded. Taken together, a typical feature of cases with thrombocytopenia must guard against CMV infection as the source.
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